Fibroblast interaction with carboxymethylchitosan-based hydrogels.
作者： Wanida Janvikul , Paweena Uppanan , Boonlom Thavornyutikarn , Rujiporn Prateepasen , Somporn Swasdison
DOI: 10.1007/S10856-006-0099-5
关键词: Chitosan 、 Cell aggregation 、 Materials science 、 Steaming 、 Scanning electron microscope 、 Viscosity 、 Polymer 、 Polymer chemistry 、 Vinyl alcohol 、 Chemical engineering 、 Self-healing hydrogels
摘要: The interaction between L929 cells and carboxymethylchitosan (CM-chitosan)-based hydrogels, hydrogels from pure CM-chitosan its blends, was examined in this study. Cytotoxicity of all materials also assessed. cellular morphology behavior on the surfaces were observed by scanning electron microscopy (SEM). effects various parameters, e.g., type content blended polymers, surface structure steaming condition used for preparation cell-material response investigated. results cytotoxicity test revealed that non-cytotoxic. SEM micrographs demonstrated proliferated spread onto a porous sample. Better cell spreading found flat film. Rounded when poly(vinyl alcohol) (PVA) incorporated into CM-chitosan. Fewer PVA increased. Spherical clusters aggregated existed blends with ultra high viscosity carboxymethylcellulose (CM-cellulose). In contrast, use low CM-cellulose, appeared more spreading. attached film steamed at highest temperature longest period to most among tested conditions.
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