Uptake of thyroid hormones in neonatal rat cardiac myocytes.

摘要: The uptake and metabolism of T3 T4 were investigated in cardiomyocytes isolated from 2-day-old rats. Myocytes (2-5 x 10(5) cells/well) cultured for 1 day medium with 5% horse serum-5% FCS subsequently 4 days without serum; some cases myocytes serum throughout the culture period. Experiments performed at 37 C 0.5% BSA measurement [125I]T3 (200,000 cpm; 200 pM) 0.1% [125I]T4 350 uptake. Uptake [125I]T3, expressed as femtomoles per picomolar concentration free hormone, any incubation time between 15 min 24 h was least 2-fold higher than that [125I]T4. Neither nor deiodinated within h. This observed cells absence or presence serum. After incubation, 0.048 +/- 0.002 fmol/pM (n = 9), 0.018 0.003 9). Although reduced by 31-4...