作者: K Ojamaa , J D Klemperer , S S MacGilvray , I Klein , A Samarel
DOI: 10.1210/ENDO.137.3.8603588
关键词:
摘要: Thyroid hormone exerts marked effects on cardiovascular function. Expression of cardiac alpha- and beta-myosin heavy chain (MHC) isoforms can be altered in response to thyroid as well by hemodynamic changes imposed the heart. The molecular mechanisms that mediate these are not completely known. We studied contractile responsiveness betaMHC promoter both cultured myocytes vivo direct DNA transfer. Using transient transfection neonatal rat cardiomyocytes, activities recombinant reporter plasmids containing 5'-flanking sequences terminating at positions -2250, -1145, -670, -354 were decreased significantly cultures L-T3 (50 nM). Similar deletion analysis showed regions within -2250 -151 bp contractility responsive; however, position -126 attenuated this response. In activity, determined injecting plasmid into th...