The novel agent ophiobolin O induces apoptosis and cell cycle arrest of MCF-7 cells through activation of MAPK signaling pathways.

作者: Tingting Yang , Zhenyu Lu , Li Meng , Shanjian Wei , Kui Hong

DOI: 10.1016/J.BMCL.2011.10.079

关键词: KinaseMCF-7MAPK/ERK pathwayChemistryApoptosisAnnexinMitogen-activated protein kinaseCell cycle checkpointCell biologyp38 mitogen-activated protein kinases

摘要: Abstract Ophiobolin O is a natural compound that has been isolated from Aspergillus ustus 094102. This the first study to demonstrate anti-proliferative effect of ophiobolin in human breast cancer MCF-7 cells. The results present show induced cycle G0/G1 phase arrest cells using cell analysis. In addition, we demonstrated reduced viability time- and dose-dependent manner efficiently apoptosis Annexin V/PI binding assay. also caused activation JNK (c-Jun NH2-terminal kinase), p38 MAPK (mitogen activated protein kinase) ERK (extracellular signal-regulated as well degradation Bcl-2 phosphorylation (Ser70). Bax expression was not changed O-treated Taken together, may be considered novel therapeutic agent cancer.

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