Individual expression features of GPX2, NQO1 and SQSTM1 transcript variants induced by hydrogen peroxide treatment in HeLa cells.

摘要: Pathway activity assessment-based approaches are becoming highly influential in various fields of biology and medicine. However, these mostly rely on analysis mRNA expression, total from a given locus is measured the majority cases. Notably, significant portion protein-coding genes produces more than one transcript. This biological fact responsible for noise when changes transcription single gene analyzed. The NFE2L2/AP-1 pathway an attractive target biomedical applications. To date, there lack data regarding agreement expression even classical this pathway. In present paper we analyzed whether transcript variants GPX2, NQO1 SQSTM1 were characterized by individual features HeLa cells exposed to pro-oxidative stimulation with hydrogen peroxide. We found that all transcripts (10 total) appeared be significantly individually regulated under conditions tested. conclude transcripts, rather mRNA, best markers activation. also discuss here some roles regulation.