The single-step method of RNA isolation by acid guanidinium thiocyanate–phenol–chloroform extraction: twenty-something years on

作者: Piotr Chomczynski , Nicoletta Sacchi

DOI: 10.1038/NPROT.2006.83

关键词:

摘要: … RNA isolation became almost imperative for accomplishing our research projects. The discovery that mostly total RNA … Twenty years ago, total RNA extracted with our protocol was used …

参考文章(10)
William W. Wilfinger, Karol Mackey, Piotr Chomczynski, Effect of pH and ionic strength on the spectrophotometric assessment of nucleic acid purity BioTechniques. ,vol. 22, pp. 474- 481 ,(1997) , 10.2144/97223ST01
ED Harlow, David Lane, A Laboratory manual Cold Spring Harbor Laboratory. ,(1989)
Piotr Chomczynski, Product and process for isolating RNA ,(1987)
John M Chirgwin, Alan E Przybyla, Raymond J MacDonald, William J Rutter, None, Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry. ,vol. 18, pp. 5294- 5299 ,(1979) , 10.1021/BI00591A005
Piotr Chomczynski, Solubilization in formamide protects RNA from degradation Nucleic Acids Research. ,vol. 20, pp. 3791- 3792 ,(1992) , 10.1093/NAR/20.14.3791
Piotr Chomczynski, Nicoletta Sacchi, Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction Analytical Biochemistry. ,vol. 162, pp. 156- 159 ,(1987) , 10.1016/0003-2697(87)90021-2
Tom Maniatis, Joseph Sambrook, E. F. Fritsch, Molecular Cloning: A Laboratory Manual ,(2001)
Pamela Knight, DNA Sequencing Markets Nature Biotechnology. ,vol. 8, pp. 148- 148 ,(1990) , 10.1038/NBT0290-148