Deep Amplicon Sequencing Quantitatively Detected Mixed Community Assemblages of Symbiodinium in Orbicella faveolata and Orbicella franksi

摘要: Author(s): Green, Elizabeth | Advisor(s): Medina, Monica; Barlow, Miriam Abstract: The taxonomic diversity of endosymbiont Symbiodinium species and the physiological contributions they confer to coral host are challenging assess. It seems likely genetic obligate modulates performance reef building corals. Here we used deep amplicon sequencing reveal high endosymbiotic dinoflagellate, Symbiodinium, in hosts by amplifying ribosomal DNA internal transcribed spacer region 2 (ITS-2). We revealed quantitative proportions mixed populations within Orbicella complex collected from three Caribbean locations. at Flower Garden Banks for franksi faveolata demonstrated contained clade B type B1 detected five genetically variable types, which may be endemic Our second dataset assessed O. Puerto Morelos, Mexico, previously genotyped using restriction fragment length polymorphism (RFLP) direct sequencing. an additional background population A, A13. Additionally showed one colony hosted a infection genotypes A13 where RFLP assigned C7 B1. third across depth gradient Curacao Illumina MiSeq platform. shallow water more relative deeper waters genotype G3, not commonly associated with scleractinian hosts. These results highlight need consistent molecular genotyping techniques assess community assemblages Symbiodinium-host relationships. This approach characterize cryptic will potentially contribute understanding variations among populations.