Interactions stéroïdes-protéines dans le sérum de l'Homme, d'un Amphibien et d'un Cyclostome: Chromatographie analytique sur échangeurs d'ions
作者： G.A. Boffa , B. Martin , J.J. Winchenne , R. Ozon
DOI: 10.1016/S0300-9084(72)80018-X
关键词: Steroid 、 Biochemistry 、 Hormone 、 Testosterone 、 Transferrin 、 Globulin 、 Sex steroid 、 Corticosterone 、 Biology 、 Transcortin
摘要: Summary 1. Steroid-protein interactions in Amphibians and Cyclostoma sera are studied by analytical ion exchange chromatography electrophoresis associated to autoradiography. As higher vertebrates these species possess proteins with high steroid affinity. 2. Our results confirmed that three binding occured human sera. SBP is a β globulin which possesses affinity for testosterone estradiol-17 β. CBG or α 1 -α 2 smaller than compounds. 3. Two present Pleurodele (Amphibian). One sex (SBP). The second one low concentration binds corticosterone. This protein can be compared the mammalian transcortin. electronegative charge of this weaker according its DEAE cellulose behaviour. transferrin distinguished electrophoretic chromatographic patterns. 4. also found Lamprey (Cyclostoma) : an globulin. Progesterone show marked two components but progesterone preferentially bound In specificity less restrictive even Amphibian SBP. clearly separated from transferrin. — corticosterone complexes more affected temperature those formed progesterone. able fo bind hormones, seems wider Albumine-α third system it revealed autoradiography [ 14 C].
sci-hub.se 参考文章(24)
George L. Selden, Ulrich Westphal, Billy D. Ashley, Steroid-protein interactions: VII. Interactions of progesterone and corticosteroids with human plasma proteins determined by multiple equilibrium dialysis Archives of Biochemistry and Biophysics. ,vol. 92, pp. 441- 448 ,(1961) , 10.1016/0003-9861(61)90383-6
Ulrich Westphal, Steroid-protein interactions Archives of Biochemistry and Biophysics. ,vol. 118, pp. 556- 567 ,(1967) , 10.1016/0003-9861(67)90390-6
Thomas G. Muldoon, Ulrich Westphal, Steroid-protein interactions. XV. Isolation and characterization of corticosteroid-binding globulin from human plasma. Journal of Biological Chemistry. ,vol. 242, pp. 5636- 5643 ,(1967) , 10.1016/S0021-9258(18)99404-4
J L Guériguian, W H Pearlman, Some Properties of a Testosterone-binding Component of Human Pregnancy Serum Journal of Biological Chemistry. ,vol. 243, pp. 5226- 5233 ,(1968) , 10.1016/S0021-9258(18)91941-1
W H Pearlman, I F F Fong, J H Tou, A Further Study of a Testosterone-binding Component of Human Pregnancy Serum Journal of Biological Chemistry. ,vol. 244, pp. 1373- 1380 ,(1969) , 10.1016/S0021-9258(18)91852-1
William H. Pearlman, Odette Crépy, Steroid-Protein Interaction with Particular Reference to Testosterone Binding by Human Serum Journal of Biological Chemistry. ,vol. 242, pp. 182- 189 ,(1967) , 10.1016/S0021-9258(19)81446-1
A. Lewis Farr, Oliver H. Lowry, Rose J. Randall, Nira J. Rosebrough, Protein Measurement with the Folin Phenol Reagent Journal of Biological Chemistry. ,vol. 193, pp. 265- 275 ,(1951)
George Scatchard, The Attractions of Proteins for Small Molecules and Ions Annals of the New York Academy of Sciences. ,vol. 51, pp. 660- 672 ,(1949) , 10.1111/J.1749-6632.1949.TB27297.X
Jean-Pierre Raynaud, Christine Mercier-Bodard, Etienne E. Baulieu, Rat estradiol binding plasma protein (EBP). Steroids. ,vol. 18, pp. 767- 788 ,(1971) , 10.1016/0039-128X(71)90035-3
R. Ozon, B. Martin, G.A. Boffa, Protein binding of estradiol and testosterone in newt serum (Pleurodeles waltlii Michah.) General and Comparative Endocrinology. ,vol. 17, pp. 566- 570 ,(1971) , 10.1016/0016-6480(71)90192-4