Molecular cytogenetic characterization of chromosome 9-derived material in a human thyroid cancer cell line.

摘要: The incidence of papillary thyroid carcinoma (PTC) increases significantly after exposure the head and neck region to ionizing radiation, yet we know neither steps involved in malignant transformation epithelium nor specific carcinogenic mode action radiation. Such increased tumor frequency became most evident children 1986 nuclear accident Chernobyl, Ukraine. In eight years following accident, average childhood PTCs (chPTC) 70-fold Belarus, 200-fold Gomel, 10-fold Ukraine 50-fold Tschnigov, Kiev, Rovno, Shitomyr Tscherkassy compared rate about 1 per 106 year prior (Likhtarev et al., 1995; Sobolev 1997; Jacob 1998). To study etiology radiation-induced cancer, formed an international consortium investigate chromosomal changes altered gene expression cases post-Chernobyl chPTC. Our approach is based on karyotyping primary cultures established from chPTC specimens, establishment cell lines studies genotype-phenotype relationships through high resolution chromosome analysis, DNA/cDNA micro-array studies, mouse xenografts that test for tumorigenicity. Here, report application fluorescence situ hybridization (FISH)-based techniques molecular cytogenetic characterization a highly tumorigenic line, S48TK, its subclones. Using 9 rearrangements as example, describe new termed ‘BAC-FISH’ rapidly delineate breakpoints, important step towards better understanding formation translocations their functional consequences.