Genetic aberrations detected by comparative genomic hybridization predict outcome in node-negative breast cancer

摘要: Breast cancer progression is determined by a complex pattern of multiple genetic aberrations the association which with patient prognosis unknown. In this study, we have undertaken genome-wide screening to detect changes associated clinical outcome in node-negative breast cancer. Comparative genomic hybridization was used screen for DNA sequence gains and losses across all human chromosomes 23 tumors from patients no disease recurrence after at least 5 years follow-up 25 during first follow-up. The total number (copy losses) per tumor significantly greater group (P = 0.019) subgroup these who died as result 0.0022). When copy were analyzed separately, only significant 0.013 P 0.002 overall survival). Of individual loci involved, high level gain long arm chromosome 8 0.01, Fisher's exact test). Furthermore, amplification sequences 20q12-13 found 7 cases (15%), 6 had early within 32 months diagnosis. This overview comparative suggests that genetically advanced cancers having may poor increased two specific regions, 8q 20q13, confer more aggressive phenotype. Results pilot study suggest determination help therapeutic decision making. Specific probes should be developed test prognostic value amplifications large numbers patients.