Detection and mapping of amplified DNA sequences in breast cancer by comparative genomic hybridization.

摘要: Comparative genomic hybridization was applied to 5 breast cancer cell lines and 33 primary tumors discover map regions of the genome with increased DNA-sequence copy-number. Two-thirds almost all showed copy-number affecting a total 26 chromosomal subregions. Most these loci were distinct from those currently known amplified genes in cancer, sequences originating 17q22-q24 20q13 showing highest frequency amplification. The results indicate that may contain previously unknown whose expression contributes progression. Chromosomal often spanned tens Mb, suggesting involvement more than one gene each region.