Molecular Genetic Studies of Chromosome 8 and Oncogene c-myc in Breast Cancer
作者： Jaana Rummukainen
DOI:
关键词: Oncology 、 Comparative genomic hybridization 、 Breast cancer 、 Chromogenic in situ hybridization 、 Cancer research 、 Molecular pathology 、 CISH 、 Karyotype 、 Biology 、 Internal medicine 、 Fluorescence in situ hybridization 、 Oncogene
摘要: 8 INTRODUCTION 11 REVIEW OF THE LITERATURE 13 1. Breast cancer 1.1. Epidemiology 1.2. Pathogenesis of breast 2. Methods used in the genomic studies 15 2.1. Studies on aberrations 16 2.2. Comparative using multiple techniques 18 3. Molecular pathology 19 4. Gene amplifications 21 5. Tumor suppressor genes 23 6. Chromosome 26 6.1. The central role c-myc cellular functions 6.2. proto-oncogene 28 AIMS STUDY 31 4 MATERIALS AND METHODS 32 cell lines (I, II) Primary patients and tumor samples (III, IV) hybridization 33 Spectral karyotyping (SKY) 34 4.1. Comparison CGH SKY data (I) 35 Fluorescence situ (I-IV) 5.1. Probes 5.2. Procedure 36 5.3. FISH microscopy 37 Chromogenic (CISH) (IV) 38 7. Statistical methods 39 RESULTS 40 Chromosomal alterations 1.3. Combination CGH, 43 Structural chromosome 44 Imbalances by Translocations arm-specific painting amplification 45
researchgate.net 参考文章(90)
Karhu R, Tanner M, Pennanen S, Kuukasjärvi T, Kallioniemi Op, Isola J, Genetic changes in intraductal breast cancer detected by comparative genomic hybridization. American Journal of Pathology. ,vol. 150, pp. 1465- 1471 ,(1997)
F. M. Waldman, S. G. Hilsenbeck, C. K. Osborne, S. A. W. Fuqua, O.-P. Kallioniemi, J. J. Isola, L. W. Chu, Genetic aberrations detected by comparative genomic hybridization predict outcome in node-negative breast cancer American Journal of Pathology. ,vol. 147, pp. 905- 911 ,(1995)
Bruno Amati, Konstantinos Alevizopoulos, Jaromir Vlach, Myc and the cell cycle Frontiers in Bioscience. ,vol. 3, pp. d250- 268 ,(1998) , 10.2741/A239
J. Michael Bishop, Retroviruses and Cancer Genes Advances in Cancer Research. ,vol. 37, pp. 1- 32 ,(1982) , 10.1016/S0065-230X(08)60880-5
Helen Donis-Keller, Nancy J. Phillips, Keri L. Fair, Matthew Holt, Diane M. Radford, Jon H. Ritter, Allelic Loss and the Progression of Breast Cancer Cancer Research. ,vol. 55, pp. 5180- 5183 ,(1995)
Christoph Lengauer, Kenneth W. Kinzler, Bert Vogelstein, Genetic instabilities in human cancers Nature. ,vol. 396, pp. 643- 649 ,(1998) , 10.1038/25292
Peter A Jones, Peter W Laird, Cancer-epigenetics comes of age Nature Genetics. ,vol. 21, pp. 163- 167 ,(1999) , 10.1038/5947
Robert Zeillinger, Geneviève Louason, Frank Courjal, Paul Speiser, Charles Theillet, Carmen Rodriguez, Joelle Simony-Lafontaine, Marguerite Cuny, Mapping of DNA amplifications at 15 chromosomal localizations in 1875 breast tumors: definition of phenotypic groups. Cancer Research. ,vol. 57, pp. 4360- 4367 ,(1997)
Bert Vogelstein, Kenneth W. Kinzler, The Genetic Basis of Human Cancer ,(1997)
Bernard Dutrillaux, Michele Gerbault-Seureau, Brigitte Zafrani, Characterization of chromosomal anomalies in human breast cancer Cancer Genetics and Cytogenetics. ,vol. 49, pp. 203- 217 ,(1990) , 10.1016/0165-4608(90)90143-X