How to Build an Integrated Biobank: The Washington University Translational Cardiovascular Biobank & Repository Experience

摘要: Biomedical research has undergone a paradigm shift since sequencing of the human genome opened door to deeper understanding molecular basis disease and spawned an explosion in cutting edge technologies applied elucidating mechanisms specifically relevant states. Despite enormous invaluable contribution that experimental animals have made our both physiology pathogenesis humans, it become increasingly clear pathogenesis, development potential treatment strategies therapeutic efficacy would, at some point preclinical investigatory pathway, be best assessed human, as opposed animal, tissues. Human tissue sampling is also imperative for fulfilling promise personalized medicine realized from advances insights generated next-generation sequencing1 disease- patient-specific induced pluripotent stem cells.2 Biospecimen science emerging multidisciplinary field dedicated establishing procedures collection, processing, shipping storage biospecimens been experimentally tested rigorously validated limit alterations quality, composition consistency collected samples.3, 4 Several outstanding reports emanating organizations such International Society Biological Environmental Repositories (ISBER)5 National Cancer Institute’s (NCI’s) Office Biorepositories Biospecimen Research (OBBR)6 delineated published methods practices sample quality control documentation should consulted their wealth requisite considerations.7,8 The purpose this article detail parameters we found critical success maintenance integrated cardiovascular biospecimen repository, Translational Cardiovascular Biobank & Repository (TCBR), established Washington University School Medicine. The TCBR was founded acquisition (using standardized, procedures), utilization tissues detailed phenotypic (electrophysiological, structural, biochemical) genotypic (genomic, epigenetic somatic) analyses. Our repository houses collection tissues, including blood products, fully with clinical information patients or individuals who donated tissue, well data samples. A number institutions heart biobanks these include Harvard Tissue Bank (http://cardiogenomics.med.harvard.edu/component-detail?project_id=236), Pennsylvania Heart (http://somapps.med.upenn.edu/ohr/card/viewpub.php?rc=Nt most mature entity pediatric Centre Hospital Sick Children Toronto (http://www.heartcentrebiobank.ca/home/index.php). However, despite increasing banks being established, specific, practical “instruction manual” outlining each required elements successful biobank replete regulatory considerations, specific infrastructure recommendations, pitfalls problems avoid, does not exist.