Effect of Acinetobacter sp on Metalaxyl Degradation and Metabolite Profile of Potato Seedlings (Solanum tuberosum L.) Alpha Variety

作者: Fabiola G. Zuno-Floriano , Marion G. Miller , Maria L. Aldana-Madrid , Matt J. Hengel , Nilesh W. Gaikwad

DOI: 10.1371/JOURNAL.PONE.0031221

关键词: AcinetobacterPhytophthora infestansBotanySeedlingBiologyCultivarInoculationMetalaxylRhizosphereFungicide

摘要: One of the most serious diseases in potato cultivars is caused by pathogen Phytophthora infestans, which affects leaves, stems and tubers. Metalaxyl a fungicide that protects plants from infestans. In Mexico, farmers apply metalaxyl 35 times during cycle production last application typically 15 days before harvest. There are no records related to presence tubers Mexico. present study, we evaluated effect Acinetobacter sp on degradation seedlings. The bacteria growth seedlings was also evaluated. A metabolite profile analysis conducted determine potential molecular biomarkers produced metalaxyl. did not affect However, strongly affected inoculated seedlings, as confirmed plant length fresh weights were lower (40% 27%, respectively) compared controls. root formation. Inoculated showed decrease formation LC-MS/MS residues suggests degrade GC–TOF–MS platform used metabolic profiling studies. Statistical data pathway allowed suggesting alteration pathways both infection treatment. Several hundred metabolites detected, 137 identified markers suggested based statistical change significance found with PLS-DA analysis. These results important for better understanding interactions putative endophytic pesticides their possible effects metabolism.

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