Prenatal dexamethasone rescues heart hypoplasia in fetal rats with congenital diaphragmatic hernia
作者： L. Migliazza , H.M. Xia , A. Arnaiz , J.I. Alvarez , L.F. Alfonso
关键词: Nitrofen 、 Hypoplasia 、 Respiratory muscle 、 Internal medicine 、 Heart disease 、 Dexamethasone 、 Medicine 、 Fetus 、 Congenital diaphragmatic hernia 、 Corticosteroid 、 Endocrinology 、 Pediatrics, Perinatology, and Child Health 、 Surgery 、 General Medicine
摘要: Abstract Background/Purpose: Patients and rats with congenital diaphragmatic hernia (CDH) have lung heart hypoplasia. Prenatal steroids improve hypoplasia in CDH rats. The current study tests the hypothesis that prenatal dexamethasone could rescue CDH. Methods: Timed pregnant received intragastrically either 100 mg nitrofen or oil on day 9.5, other animals had same treatment with, addition, 0.25 mg/kg intraperitoneally no days 19 20. Fetuses were recovered 21, weight to body ratios, DNA, protein, glycogen measured fresh specimens. Left-to-right ventricular diameter aortic-to-pulmonary ratios after formalin fixation. Results: Wet weight, left-to-right diameter, root which lower fetuses exposed only than their controls, similar those plus corresponding controls. Total was decreased comparison increased receiving theirs. Protein DNA ratio all irrespective of exposure not dexamethasone. Glycogen higher dexamethasone-treated without this treatment. No gross histologic differences seen among groups. Conclusions: Heart is part rescued by as expressed number smaller myocytes content. modify involvement human well. J Pediatr Surg 35:1757-1761. Copyright © 2000 W.B. Saunders Company.
elsevier.com
sci-hub.se 参考文章(32)
Duncan T. Wilcox, Michael S. Irish, Bruce A. Holm, Philip L. Glick, Pulmonary Parenchymal Abnormalities in Congenital Diaphragmatic Hernia Clinics in Perinatology. ,vol. 23, pp. 771- 779 ,(1996) , 10.1016/S0095-5108(18)30208-2
K K Nobuhara, J M Wilson, Pathophysiology of congenital diaphragmatic hernia. Seminars in Pediatric Surgery. ,vol. 5, pp. 234- 242 ,(1996)
Knuppel Ra, Angel Jl, Morales Wj, Sawai S, O'Brien Wf, Fetal lung maturation: the combined use of corticosteroids and thyrotropin-releasing hormone. Obstetrics & Gynecology. ,vol. 73, pp. 111- 116 ,(1989)
B. Thébaud, A. Azancot, P. de Lagausie, E. Vuillard, L. Ferkadji, K. Benali, F. Beaufils, Congenital diaphragmatic hernia: antenatal prognostic factors Intensive Care Medicine. ,vol. 23, pp. 1062- 1069 ,(1997) , 10.1007/S001340050457
S Lo, J C Russell, A W Taylor, Determination of glycogen in small tissue samples. Journal of Applied Physiology. ,vol. 28, pp. 234- 236 ,(1970) , 10.1152/JAPPL.1970.28.2.234
R. N. Howie, G. C. Liggins, A CONTROLLED TRIAL OF ANTEPARTUM GLUCOCORTICOID TREATMENT FOR PREVENTION OF THE RESPIRATORY DISTRESS SYNDROME IN PREMATURE INFANTS Pediatrics. ,vol. 50, pp. 515- 525 ,(1972)
Alejandro Losada, Juan A. Tovar, Hui M. Xia, Juan A. Diez-Pardo, Pilar Santisteban, Down-regulation of thyroid transcription factor-1 gene expression in fetal lung hypoplasia is restored by glucocorticoids. Endocrinology. ,vol. 141, pp. 2166- 2173 ,(2000) , 10.1210/ENDO.141.6.7522
Hratch L. Karamanoukian, Stuart J. O'Toole, Jon R. Rossman, Anjmun Sharma, Richard G. Azizkhan, Philip L. Glick, Fetal Surgical Interventions and the Development of the Heart in Congenital Diaphragmatic Hernia Journal of Surgical Research. ,vol. 65, pp. 5- 8 ,(1996) , 10.1006/JSRE.1996.0335
Jay M Wilson, Dennis P Lund, Craig W Lillehei, Joseph P Vacanti, Congenital diaphragmatic hernia--a tale of two cities: the Boston experience. Journal of Pediatric Surgery. ,vol. 32, pp. 401- 405 ,(1997) , 10.1016/S0022-3468(97)90590-X
Cesar Labarca, Kenneth Paigen, A simple, rapid, and sensitive DNA assay procedure. Analytical Biochemistry. ,vol. 102, pp. 344- 352 ,(1980) , 10.1016/0003-2697(80)90165-7