Distinct cytoplasmic regions of the growth hormone receptor are required for activation of JAK2, mitogen-activated protein kinase, and transcription.

摘要: The GH receptor (GHR) is a member of the cytokine/hematopoietic growth factor family, and protein tyrosine phosphorylation has been implicated in signaling cascade these receptors. It was recently shown that kinase JAK2 associated with GHR. induces activation JAK2, which phosphorylates itself receptor. Mitogen-activated (MAP) transcriptional stimulation specific genes, such as Spi 2.1, have also reported to be induced by GH. To identify functionally important regions cytoplasmic domain GHR, we compared actions wild-type receptor, two truncated mutants, one internal deletion mutant (similar intermediate Nb2 form PRL receptor) transfectants Chinese hamster ovary cell line. A region 46 amino acids adjacent membrane found sufficient for both MAP kinases. This contains proline-rich sequence (box 1) conserved cytokine r...