SuReSim: simulating localization microscopy experiments from ground truth models
作者： Varun Venkataramani , Frank Herrmannsdörfer , Mike Heilemann , Thomas Kuner
DOI: 10.1038/NMETH.3775
关键词: Data mining 、 Software tool 、 Software 、 Image processing 、 Super-resolution microscopy 、 Ground truth 、 Computer science 、 Microscopy 、 Research question
摘要: Super-resolution fluorescence microscopy has become a widely used tool in many areas of research. However, designing and validating super-resolution experiments to address research question technically feasible scientifically rigorous manner remains fundamental challenge. We developed SuReSim, software that simulates localization data arbitrary three-dimensional structures represented by ground truth models, allowing users systematically explore how changing experimental parameters can affect potential imaging outcomes.
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