Spread and predominance in Japan of novel G1P[8] double-reassortant rotavirus strains possessing a DS-1-like genotype constellation typical of G2P[4] strains

摘要: Rotavirus is a major cause of severe gastroenteritis in children <5 years age worldwide, and two, live attenuated rotavirus vaccines are globally available. As introduced into national immunization programs, there an increasing need to monitor circulating wild-type strains. However, few studies have systematically examined their full genotype constellation. This study was therefore undertaken characterize the whole constellation strains three widely-separated locations Japan during 2012 season when became available country for first time. Of 107 rotavirus-positive specimens, 50 (46.7%) collected from all possessed unusual G1-P[8]-I2-R2-C2-M2-A2-N2-T2-E2-H2 which typical G2P[4] strain appeared acquired its two surface protein genes most common G1P[8] strain. These double-reassortant were shown possess 11 genome segments virtually indistinguishable each other nucleotide sequences phylogenetic lineages except that underwent further intra-genotype reassortment. Successful spread predominance broad across novel possessing previously thought not be preferred suggests unexpected genomic flexibility