Cytogenetic characterization of an established xenografted prostatic adenocarcinoma cell line (PC-82).
作者： Josée J. König , Anne Hagemeijer , Bep Smit , Eric Kamst , Johan C. Romijn
DOI: 10.1016/0165-4608(88)90173-2
关键词: Carcinoma 、 Genetics 、 Prostatic adenocarcinoma 、 Preparation method 、 Karyotype 、 Molecular biology 、 Cell culture 、 Biology
摘要: Detailed cytogenetic analysis was performed of a xenografted human prostatic adenocarcinoma cell line PC-82. A direct preparation method developed that yielded metaphases good quality. Flow cytometric data and banding showed near-tetraploid karyotype with 18 consistent marker chromosomes. As result the rearrangements involved, parts chromosomes 2, 3, 4, 7, 9, 10, 15, 18, 21 were homozygous, while regions on 2p, 13q, 17q apparently completely lost. In contrast to this, some #2, #5, and, especially, #1 present in three or even four times normal copy number. Comparison affected PC-82 all available carcinoma 1, 6, 15 be involved over 50% carcinomas. When only from primary adenocarcinomas (including PC-82) taken into account it appeared 10 five tumors studied.
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