Conditioned medium from normoxia (WJMSCs-norCM) and hypoxia-treated WJMSCs (WJMSCs-hypoCM) in inhibiting cancer cell proliferation

摘要: Mesenchymal stem cells (MSCs) have unique properties, including high proliferation rates, self-renewal, multilineage differentiation ability, wide multipotency, hypoimmunogenicity, noninduction of teratomas, and anticancer properties. MSCs can be isolated from embryonic and extraembryonic tissues as well adult organs. Human Wharton’s jelly cellconditioned medium possesses properties inhibits the growth solid tumors. Lower oxygen concentration or hypoxic condition increase proliferation MSCs, but there are no differences in surface markers. We determined osteocyte, chondrocyte, and adipocyte normoxic WJMSCs (nor-WJMSCs) 2.5%, hypoxic 5% (hypo-WJMSCs); a different passage (P4 P8), we inhibitory effect WJMSCs-norCM WJMSCs-hypoCM on human cancer cells including cervical (HeLa), liver (HepG2), prostate (pc3), ovarian (skov3), oral squamous (hsc3) cell lines compared to normal mouse fibroblast (NIH3T3), fibroblast, mesenchymal (hMSCs). Surfacer marker expression nor- WJMSCs-and hypo-WJMSCs P4 P8 were >95% for CD90, CD73 CD105 <2% for CD14, CD19, CD34, CD45, HLDA-II. Nor-WJMSCs underwent differentiation chondrocyte, adipocyte. and WJMSCs-hypoCM could inhibit various with minimum inhibitory concentration (IC50) 51.690e81.440% cause low inhibition IC50 136.290e185.339%. not cytotoxic toward normal cells. showed significant in MSC differentiation. could inhibit lines, safe cells.