Deactivation mechanism of the green fluorescent chromophore.

摘要: We report time-resolved fluorescence data for the anion of p-hydroxybenzylidene dimethylimidazolinone (p-HBDI), a model chromophore green protein, in viscous glycerol−water mixtures over range temperatures, T. The markedly nonexponential decay excited electronic state is interpreted with aid an inhomogeneous possessing Gaussian coordinate-dependent sink term. A nonlinear least-squares fitting routine enables us to achieve quantitative fits by adjusting single activation parameter, which found depend linearly on 1/T. derive analytic expression absolute quantum yield, compared integrated steady-state spectra. microscopic origins are discussed terms two-dimensional dynamics, coupling phenyl-ring rotation swinging mode that brings this flexible molecule proximity conical intersection its multidimensional potential energy surface.