Meta and para effects in the ultrafast excited-state dynamics of the green fluorescent protein chromophores.
作者： Kyril M. Solntsev , Olivier Poizat , Jian Dong , Julien Rehault , Yongbing Lou
DOI: 10.1021/JP077707T
关键词:
摘要: Femtosecond transient absorption and fluorescence upconversion experiments have been performed to investigate the photoinduced dynamics of meta isomer green fluorescent protein chromophore, m-HBDI, its O-methylated derivative, m-MeOBDI, in various solvent mixtures at neutral, acidic, basic pH. The para isomer, p-HBDI, O- N-methylated derivatives, p-MeOBDI p-HBDIMe(+), were also studied for comparison. In all cases, fast quenching excited S1 state by internal conversion (IC) ground was observed. compounds, IC, presumably promoted twisting, arises <1 ps. A similar process takes place compounds nonaqueous solvents but with notably slower kinetics. aqueous solutions, undergo ultrafast intermolecular excited-state proton transfer that competes isomerization.
acs.org
sci-hub.se 参考文章(56)
Ulrich Schindewolf, Physical and Chemical Properties of Dissolved Electrons(Excess Electrons) Angewandte Chemie International Edition in English. ,vol. 17, pp. 887- 901 ,(1978) , 10.1002/ANIE.197808871
Konstantin Leonid Litvinenko, Naomi Marica Webber, Stephen Roy Meech, Ultrafast Excited State Relaxation of the Chromophore of the Green Fluorescent Protein Bulletin of the Chemical Society of Japan. ,vol. 75, pp. 1065- 1070 ,(2002) , 10.1246/BCSJ.75.1065
Pavel Leiderman, Liat Genosar, Dan Huppert, Xiaokun Shu, S. James Remington, Kyril M. Solntsev, Laren M. Tolbert, Ultrafast excited-state dynamics in the green fluorescent protein variant S65T/H148D. 3. Short- and long-time dynamics of the excited-state proton transfer. Biochemistry. ,vol. 46, pp. 12026- 12036 ,(2007) , 10.1021/BI7009053
Jian Dong, Kyril M. Solntsev, Olivier Poizat, Laren M. Tolbert, The Meta-Green Fluorescent Protein Chromophore Journal of the American Chemical Society. ,vol. 129, pp. 10084- 10085 ,(2007) , 10.1021/JA073622U
DP DeCosta, N Howell, AL Pincock, JA Pincock, S Rifai, None, 1,3-dimethoxy-5-methylene-1,3-cyclohexadiene compounds with leaving groups at C6: generation, solvolytic reactivity, and their importance in the photochemistry of 3,5-dimethoxybenzyl derivatives Journal of Organic Chemistry. ,vol. 65, pp. 4698- 4705 ,(2000) , 10.1021/JO000387O
Howard E. Zimmerman, Meta-Ortho Effect in Organic Photochemistry: Mechanistic and Exploratory Organic Photochemistry1,2 Journal of Physical Chemistry A. ,vol. 102, pp. 5616- 5621 ,(1998) , 10.1021/JP9803182
Jack Saltiel, Andrew S. Waller, Donald F. Sears, The temperature and medium dependencies of cis-stilbene fluorescence. The energetics of twisting in the lowest excited singlet state Journal of the American Chemical Society. ,vol. 115, pp. 2453- 2465 ,(1993) , 10.1021/JA00059A047
Anwar Usman, Omar F. Mohammed, Erik T. J. Nibbering, Jian Dong, Kyril M. Solntsev, Laren M. Tolbert, Excited-State Structure Determination of the Green Fluorescent Protein Chromophore Journal of the American Chemical Society. ,vol. 127, pp. 11214- 11215 ,(2005) , 10.1021/JA0520520
Kyril M. Solntsev, Caroline E. Clower, Laren M. Tolbert, Dan Huppert, 6-hydroxyquinoline-N-oxides: a new class of "super" photoacids. Journal of the American Chemical Society. ,vol. 127, pp. 8534- 8544 ,(2005) , 10.1021/JA0514545
Alexander A. Voityuk, Andreas D. Kummer, Maria-Elisabeth Michel-Beyerle, Notker Rösch, Absorption spectra of the GFP chromophore in solution: comparison of theoretical and experimental results Chemical Physics. ,vol. 269, pp. 83- 91 ,(2001) , 10.1016/S0301-0104(01)00334-2