Number and organization of actin-related sequences in the mouse genome
作者： Adrian J. Minty , Serge Alonso , Jean-Louis Guénet , Marganet E. Buckingham
DOI: 10.1016/S0022-2836(83)80035-7
关键词: Genome 、 Nucleic acid thermodynamics 、 Recombinant DNA 、 Biology 、 Complementary DNA 、 Pseudogene 、 Molecular biology 、 genomic DNA 、 Genetics 、 Gene 、 Plasmid
摘要: Recombinant plasmids containing cDNA sequences complementary to the two mouse striated-muscle actin messenger RNAs (pAF81, pAM91) and a non-muscle mRNA (pAL41) have been used examine number organization of actin-related in genome. A large (greater than 20) are detected on Southern blots restricted DNA, majority which hybridize both 5' 3' ends actin-coding sequence, even under conditions revealing only greater 80% homologous probes. More stringent washing these indicates that striated muscle actins each encoded by single genes, (beta or gamma) detects one closely related DNA. The segregation genes recombinant inbred strains shows not linked 1 centimorgan), skeletal gene is gene. Screening bank genomic cloned Charon 4A, genome much higher 20. In particular, five phages isolated representing part sub-family 20 50 similar but non-identical sequences, weakly probes (probably family pseudogenes), result recent amplification 17 X 10(3) base region
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