Crypt fission in the small intestine and colon. A mechanism for the emergence of G6PD locus-mutated crypts after treatment with mutagens.
作者： N A Wright , H S Park , R A Goodlad
DOI:
关键词: Phenotype 、 Molecular biology 、 Biology 、 Stem cell 、 Nitrosourea 、 Stem cell division 、 Carcinogenesis 、 Biochemistry 、 Crypt 、 Small intestine 、 Intestinal mucosa
摘要: In the small intestine and colon, administration of mutagens leads to emergence crypts populated by cells with a different, mutated phenotype. This is preceded transient rise in frequency partially phenotype, disappearance these occurs contemporaneously attainment plateau value wholly crypts. Here, using mutagen ethyl nitrosourea loss glucose-6-phosphate dehydrogenase staining as marker, we show that reached at between 4.6 7 weeks colon 12 same mice. Explanations for this difference have included differences stem cell cycle time single "master" or multiple occupying "niche" random after division. However, demonstrate crypt fission index, incidence fission, some four times higher than injection, propose an alternative hypothesis based on mechanism more rapid evolution colon. The should enable us predict results future experiments, namely proportional index.
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