摘要: We have investigated stem-cell organization in mouse small intestine (SI) by using a cellular marker induced somatic mutation. In intestinal whole mounts from heterozygous Dlb-1 b / mice stained with peroxidase conjugate of Dolichos biflorus agglutinin (DBA-Px), mutations stem cells result loss DBA-Px binding and so are recognizable as wholly or partly unstained crypts. The frequency these clonal patterns can be measured during the accumulation spontaneous untreated mice, after treatment ethylnitrosourea (ENU). results show that there is single infrequently dividing cell maintains epithelium each crypt through population transit cells. entire renewed approximately every 12 weeks.
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