Genome-Wide Characterization of the SloR Metalloregulome in Streptococcus mutans

摘要: Streptococcus mutans is the primary causative agent of human dental caries, a ubiquitous infectious disease for which effective treatment strategies remain elusive. We investigated 25-kDa SloR metalloregulatory protein in this oral pathogen, along with its target genes that contribute to cariogenesis. Previous studies have demonstrated manganese- and SloR-dependent repression sloABCR metal ion transport operon S. mutans. In present study, we demonstrate coordinates certain virulence attributes. Specifically, noted gene manganese-containing medium when binds promoter-proximal sequence palindromes on chromosome. applied genome-wide approach elucidate sequences reveal additional “class I” are subject SloR- manganese-dependent repression. These analyses identified 204 preceded by one or more conserved palindromic recognition elements (SREs). cross-referenced these those had previously as and/or manganese modulated microarray real-time quantitative reverse transcription-PCR (qRT-PCR) experiments. From analysis, number transcriptional upregulation such II”-type regulation dependent direct binding promoter-distal SREs. observations consistent bifunctional role metalloregulator implicate it development therapies aimed at alleviating mutans-induced caries formation.