Using a modified ferrous oxidation-xylenol orange (FOX) assay for detection of lipid hydroperoxides in plant tissue.
作者： John M. DeLong , Robert K. Prange , D. Mark Hodges , Charles F. Forney , M. Conny Bishop
DOI: 10.1021/JF0106695
关键词: Legume 、 Orange (colour) 、 Biological membrane 、 Biochemistry 、 Chemistry 、 Iodometry 、 Chromatography 、 Thiobarbituric acid 、 TBARS 、 Xylenol orange 、 Ferrous
摘要: The ferrous oxidation−xylenol orange (FOX) assay was adapted for quantifying lipid hydroperoxides (LOOHs) in plant extracts. Excised pieces of several fruit and vegetable species were exposed to 83 kJ m-2 day-1 biologically effective ultraviolet B irradiance (UV-BBE) 10−12 days induce cellular oxidation. LOOH thiobarbituric acid reactive substance (TBARS) concentrations these tissues assessed with the FOX iodometric assays former a modified TBARS latter. There generally good agreement between methods both prior following UV exposure. However, appeared have some difficulty consistently lower levels (<11 μM), whereas measured as low 5 μM. All exhibited UV-induced increases TBARS, indicating marked degree oxidation tissue segments. Compared assay, method consisten...
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sci-hub.se 参考文章(38)
Kutlay M Burat, Onur Bozkurt, Improvement of calibration curve for determining peroxide values of food lipids by the modified ferrous oxidation-xylenol orange method. Journal of AOAC International. ,vol. 79, pp. 995- 997 ,(1996) , 10.1093/JAOAC/79.4.995
G Paliyath, M. J Droillard, The mechanisms of membrane deterioration and disassembly during senescence Plant Physiology and Biochemistry. ,vol. 30, pp. 789- 812 ,(1992)
Mats Hamberg, Bengt Samuelsson, On the Specificity of the Oxygenation of Unsaturated Fatty Acids Catalyzed by Soybean Lipoxidase Journal of Biological Chemistry. ,vol. 242, pp. 5329- 5335 ,(1967) , 10.1016/S0021-9258(18)99432-9
J. Neuzil, R. Stocker, Free and albumin-bound bilirubin are efficient co-antioxidants for alpha-tocopherol, inhibiting plasma and low density lipoprotein lipid peroxidation. Journal of Biological Chemistry. ,vol. 269, pp. 16712- 16719 ,(1994) , 10.1016/S0021-9258(19)89449-8
Barry Halliwell, John M.C. Gutteridge, Role of free radicals and catalytic metal ions in human disease: an overview. Methods in Enzymology. ,vol. 186, pp. 1- 85 ,(1990) , 10.1016/0076-6879(90)86093-B
J E Kinsella, B Lokesh, R A Stone, Dietary n-3 polyunsaturated fatty acids and amelioration of cardiovascular disease: possible mechanisms. The American Journal of Clinical Nutrition. ,vol. 52, pp. 1- 28 ,(1990) , 10.1093/AJCN/52.1.1
Erich F. Elstner, Wolfgang Osswald, Mechanisms of oxygen activation during plant stress Proceedings of The Royal Society B: Biological Sciences. ,vol. 102, pp. 131- 154 ,(1994) , 10.1017/S0269727000014068
John M DeLong, Kenneth L Steffen, Lipid peroxidation and α-tocopherol content in α-tocopherol-supplemented thylakoid membranes during UV-B exposure Environmental and Experimental Botany. ,vol. 39, pp. 177- 185 ,(1998) , 10.1016/S0098-8472(97)00046-4
S.B. Turnipseed, A.J. Allentoff, J.A. Thompson, Analysis of Trimethylsilylperoxy Derivatives of Thermally Labile Hydroperoxides by Gas Chromatography-Mass Spectrometry Analytical Biochemistry. ,vol. 213, pp. 218- 225 ,(1993) , 10.1006/ABIO.1993.1412
Ken S. Wang, Tuu-jyi Chai, Reduction in omega-3 fatty acids by UV-B irradiation in microalgae Journal of Applied Phycology. ,vol. 6, pp. 415- 422 ,(1994) , 10.1007/BF02182158