Modifications of xyloglucan metabolism in azuki bean epicotyls under hypergravity conditions

作者: Kouichi Soga , Kuninori Arai , Kazuyuki Wakabayashi , Seiichiro Kamisaka , Takayuki Hoson

DOI: 10.1016/J.ASR.2006.12.011

关键词: ChemistryBiophysicsHypergravityAzuki beanCell wallMolecular massXyloglucanFucoseDepolymerizationIncubation

摘要: Abstract We examined the effects of hypergravity on both synthesis and depolymerization processes xyloglucan metabolisms. Azuki bean cuttings were incubated with [6- 3 H]- l -fucose, levels molecular size synthesized xyloglucans investigated in upper growing regions epicotyls. The amounts radioactivity incorporated into fraction increased during incubation, irrespective gravity conditions. H-Xyloglucans eluted higher mass than already present cell wall. Hypergravity at 300 g did not influence newly deposited xyloglucans. Thus, process was modified by gravitational force. On other hand, H-xyloglucans obtained from epicotyls grown 1 g shifted to low incubation period, which inhibited hypergravity. These results indicate that wall as large molecules but they then depolymerized wall, inhibition is mainly responsible for increase under

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