Hypergravity Experiments to Evaluate Gravity Resistance Mechanisms in Plants

摘要: Hypergravity generated by centrifugal acceleration is the only practical method to modify magnitude of gravitational for a sufficient duration on Earth and has been used analyze nature mechanism graviresponse, particularly gravity resistance, in plants. Plant organs are generally resistant acceleration. produced from centrifugation speeds range 10-300 × g, which easily benchtop centrifuge, often during plant experiments. After centrifugation, material fixed with suitable fixatives appropriate sample storage containers such as Chemical Fixation Bag. The then analyzed variety methods, depending purpose experiment. RNAlater(®) solution can be sequentially determining mechanical properties cell wall, RNA extraction (which necessary gene expression analysis), estimating enzyme activity wall proteins, levels well compositions polysaccharides. also directly microscopic observation cellular components cortical microtubules.