Generation and functional assessment of 3D multicellular spheroids in droplet based microfluidics platform.

摘要: Here we describe a robust, microfluidic technique to generate and analyze 3D tumor spheroids, which resembles microenvironment can be used as more effective preclinical drug testing screening model. Monodisperse cell-laden alginate droplets were generated in polydimethylsiloxane (PDMS) devices that combine T-junction droplet generation external gelation for spheroid formation. The proposed approach has the capability incorporate multiple cell types. For purposes of our study, spheroids with breast cancer lines (MCF-7 sensitive resistant) co-culture MCF-7 together fibroblast line (HS-5). device house 1000 on chip other functional analysis. Cellular viability array part was maintained two weeks by continuous perfusion complete media into device. performance models dose dependent response standard chemotherapeutic drug, doxorubicin (Dox) combination Dox paclitaxel (PCT) analyzed chip-based platform. Altogether, work provides simple novel, vitro platform generate, image uniform, monodisperse hydrogel tumors various omic studies therapeutic efficiency screening, an important translational step before vivo studies.