Dynamic behavioral and molecular changes induced by chronic stress exposure in mice

摘要: Depression is a leading cause of disabilities around the world, and underlying mechanisms involved in its pathophysiology are broad complex. Exposure to chronic stress risk factor for developing depressive-symptoms contributes cellular molecular changes precipitating emergence symptoms. In brain, excitatory neurons, inhibitory interneurons supporting astroglial cells all sensitive exposure known be impaired depression. Using an animal model stress, we assessed impact variable durations on behavioral deficits associated prefrontal cortex (PFC), brain region highly Mice were exposed up 35 days restraint weekly tests measuring anxiety anhedonia. PFC Protein RNA levels specific markers excitatory, synapses astroglia quantified using western blot qPCR, respectively. Correlation integrative network analyses used investigated different compartments. Results showed that induces anxiety-like behaviors within 7 days, while anhedonia-like observed only after days. At level, alterations many observed, particular with longer stress. Finally, correlation revealed male female mice react differently some seem more correlated males than females. Our study demonstrate dynamic can at levels, mice, exhibiting similar symptoms, have pathologies.