Membrane IL-1 induces bone resorption in organ culture.

摘要: We have studied the role of membrane-associated form IL-1 on bone resorption in vitro. Murine macrophages P388D1 cell line stimulated with LPS, subsequently fixed paraformaldehyde, induced proliferation C3H/HeJ mouse thymocytes presence a submitogenic concentration Con A. Membrane cells LPS organ cultures neonatal BALB/c calvaria. Polyclonal antibodies directed against membrane and soluble from P388D1, monospecific rabbit anti-murine rIL-1 alpha serum neutralized activity, as measured by thymocyte proliferation. In addition, these suppressed IL-1. The required direct contact between calvaria Salmon calcitonin strongly calcium release Indomethacin partially inhibited cells. Moreover, LPS-stimulated peritoneal or osteoblastic These results suggest that may significant inflammatory vivo.