Colony-stimulating factor 1 regulates CTP: phosphocholine cytidylyltransferase mRNA levels.
作者： T.G. Tessner , C.O. Rock , G.B. Kalmar , R.B. Cornell , S. Jackowski
DOI: 10.1016/S0021-9258(18)55286-8
关键词: Endocrinology 、 Cell cycle 、 Macrophage colony-stimulating factor 、 Colony-stimulating factor 、 Molecular biology 、 Protein biosynthesis 、 Cytidylyltransferase 、 Growth factor 、 Internal medicine 、 Phosphocholine 、 Biology 、 Cycloheximide
摘要: Growth factor regulation of phosphatidylcholine (PtdCho) metabolism during the G1 stage cell cycle was investigated in colony-stimulating 1 (CSF-1)-dependent murine macrophage cell-line BAC1.2F5. The transient removal CSF-1 arrested cells G1. Incorporation [3H]choline into PtdCho stimulated significantly h after growth addition to quiescent cells. Metabolic labeling experiments pointed CTP:phosphocholine cytidylyltransferase (CT) as rate-controlling enzyme for biosynthesis BAC1.2F5 amount CT mRNA increased 4-fold within 15 min and remained elevated 2 h. rise levels accompanied by a 50% increase total specific activity extracts 4 CSF-1. CSF-1-dependent elevation content neither attenuated nor superinduced inhibition protein synthesis with cycloheximide. rate turnover decreased presence indicating that message stabilization key determining mRNA. These data point abundance component factor-stimulated synthesis.
sci-hub.st 参考文章(43)
M M Billah, J C Anthes, The regulation and cellular functions of phosphatidylcholine hydrolysis. Biochemical Journal. ,vol. 269, pp. 281- 291 ,(1990) , 10.1042/BJ2690281
Mónica Lopez-Barahona, Paul L Kaplan, ME Cornet, María T Diaz-Meco, Pilar Larrodera, I Diaz-Laviada, AM Municio, Jorge Moscat, Kinetic evidence of a rapid activation of phosphatidylcholine hydrolysis by Ki-ras oncogene. Possible involvement in late steps of the mitogenic cascade. Journal of Biological Chemistry. ,vol. 265, pp. 9022- 9026 ,(1990) , 10.1016/S0021-9258(19)38806-4
Z M Yao, H Jamil, D E Vance, Choline deficiency causes translocation of CTP:phosphocholine cytidylyltransferase from cytosol to endoplasmic reticulum in rat liver. Journal of Biological Chemistry. ,vol. 265, pp. 4326- 4331 ,(1990) , 10.1016/S0021-9258(19)39567-5
B Diamond, R Halpern, S Schwarzbaum, The generation of macrophage-like cell lines by transfection with SV40 origin defective DNA. Journal of Immunology. ,vol. 132, pp. 1158- 1162 ,(1984)
O Kaplan, J S Cohen, Lymphocyte activation and phospholipid pathways : 31P magnetic resonance studies Journal of Biological Chemistry. ,vol. 266, pp. 3688- 3694 ,(1991) , 10.1016/S0021-9258(19)67849-X
R Sleight, C Kent, Regulation of phosphatidylcholine biosynthesis in mammalian cells. II. Effects of phospholipase C treatment on the activity and subcellular distribution of CTP:phosphocholine cytidylyltransferase in Chinese hamster ovary and LM cell lines. Journal of Biological Chemistry. ,vol. 258, pp. 831- 835 ,(1983) , 10.1016/S0021-9258(18)33124-7
P S Sohal, R B Cornell, Sphingosine inhibits the activity of rat liver CTP:phosphocholine cytidylyltransferase. Journal of Biological Chemistry. ,vol. 265, pp. 11746- 11750 ,(1990) , 10.1016/S0021-9258(19)38461-3
J S Sanghera, D E Vance, CTP:phosphocholine cytidylyltransferase is a substrate for cAMP-dependent protein kinase in vitro. Journal of Biological Chemistry. ,vol. 264, pp. 1215- 1223 ,(1989) , 10.1016/S0021-9258(19)85074-3
D Teegarden, E J Taparowsky, C Kent, Altered phosphatidylcholine metabolism in C3H10T1/2 cells transfected with the Harvey-ras oncogene. Journal of Biological Chemistry. ,vol. 265, pp. 6042- 6047 ,(1990) , 10.1016/S0021-9258(19)39288-9
S L Pelech, D E Vance, Regulation of rat liver cytosolic CTP: phosphocholine cytidylyltransferase by phosphorylation and dephosphorylation. Journal of Biological Chemistry. ,vol. 257, pp. 14198- 14202 ,(1982) , 10.1016/S0021-9258(19)45365-9