The generation of macrophage-like cell lines by transfection with SV40 origin defective DNA.

摘要: Two cell lines with properties of mature macrophages have been generated by transfection SV40 DNA mutated in the origin replication. One line, BAM, was derived from bone marrow cells a BALB/c mouse. The other BAC1, splenic adherent (BALB/c X A.CA) F1 Both produce lysozyme, collagenase, and esterase, bear Fc receptors, engage Fc-mediated phagocytosis. require colony-stimulating factor-1 for continued proliferation. In addition, they express Ia antigens, may be induced to secrete IL 1. This technique should make possible generation Ia-bearing diploid macrophage any strain it use this derive monocyte species which wild-type causes lytic infection.