Transcriptional responses to teflubenzuron exposure in European lobster (Homarus gammarus).

摘要: Increasing use of pharmaceutical drugs to delouse farmed salmon raises environmental concerns. This study describes an experiment carried out elucidate the molecular mechanisms antiparasitic drug teflubenzuron on a non-target species, European lobster. Juvenile lobsters (10.3±0.9 mm carapace length) were fed two environmentally relevant doses teflubenzuron, corresponding 5 and 20% standard medication (10 mg/kg day), termed low high dose in this study. After 114 days dietary exposure, whole-animal accumulation was determined. One claw from each animal collected for transcriptional analysis. Overall, exposed animals showed cumulative mortality. Six animals, treatment four dose, exoskeletal abnormalities (claw deformities or stiff walking legs). Residual levels juvenile lobster 2.7-fold higher (282 ng/g) compared (103 ng/g). The examination significant effects 21 39 studied genes. At level, anti-salmon lice impacted genes linked detoxification (cyp3a, cyp6a2, cyp302a, sult1b1, abcc4), cellular stress (hsp70, hsp90, chh), oxidative (cat, gpx3) DNA damage (p53), as well molting exoskeleton regulation (chi3l1, ecr, jhl1, chs1, ctbs, gap65, jhel-ces1) tissue (muscle exoskeleton). In conclusion, at sub-lethal can affect many claws.