Mechanism of Oncogenic Transformation by Rous Sarcoma Virus I. Intracellular Inactivation of Cell-Transforming Ability of Rous Sarcoma Virus by 5-Bromodeoxyuridine and Light

摘要: Chick embryo fibroblasts brought into stationary phase of growth by maintenance in serum-free Eagle's MEM medium were infected with the Bryan strain Rous sarcoma virus (B-RSV) and incubated for 18 hr presence 5-bromo-deoxyuridine (BUdR). The cells then allowed to resume deoxyribonucleic acid (DNA) synthesis addition an enriched F12 containing serum RSV antibody prevent spread viral infection. After 48 hr, cultures exposed various periods visible light, overlaid solid culture medium, observed appearance foci transformed cells. In treated BUdR at time infection, exposure light resulted a suppression focus formation from 50 90% experiments. Treatment before infection or on day after followed had no effect formation. which almost all infected, treatment did not result cell death. This suggests that transformation is due selective killing this but rather intracellular inactivation transforming ability proviral DNA.