Cellular requirements for the expression of IgM. Immunological memory in vitro.

摘要: Abstract In vitro culture techniques have been used to compare the direct (IgM) plaqueforming cell (PFC) response heterologous erythrocytes (RBC) by normal mouse spleen cells and from mice injected intravenously with 5 × 104 RBC ten days previously [low dose primed (LDP)]. Although LDP fail undergo a significant primary PFC response, their are capable of secondary or enhanced in vitro. The is shown be function of: (A) an increase frequency immunocompetent units (IU) due vivo priming, (B) increased number generated per IU subsequent stimulation. latter mediated through shorter doubling-time during logarithmic expansion population. Analysis nonadherent experiments indicate that two types interact response. Subsequent cocultivation suggested both these must “primed” allow induction adherent required for splenic serve as equivalent substitutes cells.