Dok4 is involved in Schwann cell myelination and axonal interaction in vitro.

摘要: The initial interaction between the Schwann cell and axon is a complex poorly understood aspect of myelination process. To investigate molecular mechanisms involved in this to identify novel genes required for myelination, we performed an RNA profiling analysis, comparing cells cultured alone or presence neurons. This led selection 30 genes, mostly upregulated on cell–axon interaction. Most identified proteins are associated with extracellular space signal transduction systems, consistent possible roles We functional analysis one these Dok4 (downstream kinase-4), which encodes membrane-associated tyrosine kinase substrate. Silencing RNA-mediated knock-down severely affected vitro myelination. Moreover, at early stages process, including axon, also migration proliferation. Finally, establishes interest our gene collection further understanding © 2010 Wiley-Liss, Inc.