Role of αvβ5 integrin receptor in endocytosis of crocidolite and its effect on intracellular glutathione levels in human lung epithelial (A549) cells
作者： Priyadarshini Pande , Tariq A. Mosleh , Ann E. Aust
DOI: 10.1016/J.TAAP.2005.07.017
关键词: Endocytosis 、 Cell culture 、 Biology 、 Intracellular 、 Cytochalasin 、 Integrin 、 Glutathione 、 Extracellular 、 Cell biology 、 A549 cell
摘要: Crocidolite, containing 27% iron by weight, is the most carcinogenic form of asbestos. Crocidolite fibers are endocytized alphavbeta5 integrin receptors in rabbit pleural mesothelial cells. We show here that crocidolite human lung epithelial (A549) cells and primary small airway (SAEC) Presence blocking antibody, P1F6, significantly reduced uptake A549 Thus, receptor involved endocytosis as well. Previously, it has been observed asbestos lead to changes intracellular redox environment, i.e. a marked decrease glutathione concentrations an increase extracellular In addition, was found be largely independent present on surface fiber. were treated with presence inhibitor cytochalasin D. Our data indicate that, upon preventing endocytosis, we able reverse total glutathione. The could also prevented monoclonal antibody P1F6. via linked
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