Evaluation of two distinct methods to quantify the uptake of crocidolite fibers by mesothelial cells.

摘要: Exposure to asbestos fibers increases the risk of mesothelioma in humans. One hypothetical carcinogenic mechanism is that may directly induce mutations mesothelial cells. Although uptake by cells recognized, methods for quantification have not been well established. In present study, we evaluated two distinct methods, using crocidolite and MeT5A method histological evaluation cell-block technique, which allows direct cross-sectional observation fibers. We found bright field with ×1000 magnification (oil-immersion) sample Kernechtrot staining was most suitable this purpose. The other flow cytometric analysis, permits a much larger number observed side scatter (SSC) increased intracellular fibers, “mean SSC ratio (treated/control)” useful quantification. could collect abundant internalized sorting. Results methodologies were correlated experiments. quantities incubation time loaded dosage, but they inversely associated cellular density culture.