Tissue-Specific Expression of Germin-Like Oxalate Oxidase during Development and Fungal Infection of Barley Seedlings

作者: B. Dumas , G. Freyssinet , K. E. Pallett

DOI: 10.1104/PP.107.4.1091

关键词: Plant defense against herbivoryOxalate oxidaseBiochemistryMicrobiologyEnzymePoaceaePathogenHordeum vulgareEscherichia coliBiologyOxalate oxidase activity

摘要: Oxalate oxidase activity was detected in situ during the development of barley seedlings. The presence germin-like oxalate confirmed by immunoblotting using an antibody directed against wheat germin produced Escherichia coli, which is shown to cross-react with (Hordeum vulgare) and enzymatic assay after electrophoresis protein extracts on polyacrylamide gels. In 3-d-old seedlings, localized epidermal cells mature region primary roots coleorhiza. After 10 d growth, detectable only Moreover, we show that induced leaves infection fungus Erysiphe graminis f. sp. hordei but not wounding. Thus, a new class proteins responds pathogen attack. We propose could have role plant defense through production H2O2.

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