Improved Purification Protocol for Oxalate Oxidase from Barley Roots
作者： P. G. Pietta , A. Calatroni , D. Agnellini , M. Pace
DOI: 10.1080/00327488208065681
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摘要: Abstract A purification scheme is described for oxalate oxidase from barley roots. This procedure involves gel filtration on Bio-Gel 0.5 m and affinity chromatography using oxalate, immobilized Affi-Gel 10, as a ligand. The purified enzyme was homogeneous SDS-gel electrophoresis, thin-layer electrophoresis high-performance liquid (HPLC). molecular weight of the 150.000 by specific activity 1.9 U/mg protein.
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