Structural and Spectroscopic Studies Shed Light on the Mechanism of Oxalate Oxidase
作者： Olaniyi Opaleye , Ruth-Sarah Rose , Mei M. Whittaker , Eui-Jeon Woo , James W. Whittaker
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摘要: Oxalate oxidase (EC 1.2.3.4) catalyzes the conversion of oxalate and dioxygen to hydrogen peroxide carbon dioxide. In this study, glycolate was used as a structural analogue investigate substrate binding in crystalline enzyme. The observed monodentate active site manganese ion is consistent with mechanism involving C-C bond cleavage driven by superoxide anion attack on coordinated substrate. mechanism, metal serves two functions: organize substrates (oxalate dioxygen) transiently reduce dioxygen. structure further implies important roles for specific residues (two asparagines one glutamine) correctly orientating reaction intermediates catalysis. Combined spectroscopic, biochemical, analyses mutants confirms importance asparagine organizing functional complex.
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B.G. Lane, J.M. Dunwell, J.A. Ray, M.R. Schmitt, A.C. Cuming, Germin, a protein marker of early plant development, is an oxalate oxidase. Journal of Biological Chemistry. ,vol. 268, pp. 12239- 12242 ,(1993) , 10.1016/S0021-9258(18)31377-2
MAMORU SUGIURA, HIROHIKO YAMAMURA, KAZUYUKI HIRANO, MASANORI SASAKI, MASAKO MORIKAWA, MINORU TSUBOI, Purification and Properties of Oxalate Oxidase from Barley Seedlings Chemical & Pharmaceutical Bulletin. ,vol. 27, pp. 2003- 2007 ,(1979) , 10.1248/CPB.27.2003
Zbyszek Otwinowski, Wladek Minor, Processing of X-ray diffraction data collected in oscillation mode Methods in Enzymology. ,vol. 276, pp. 307- 326 ,(1997) , 10.1016/S0076-6879(97)76066-X
Jim M. Dunwell, Richard W. Pickersgill, Eui-Jeon Woo, Peter W. Goodenough, Allison C. Marvier, Germin is a manganese containing homohexamer with oxalate oxidase and superoxide dismutase activities. Nature Structural & Molecular Biology. ,vol. 7, pp. 1036- 1040 ,(2000) , 10.1038/80954
T. Lis, Structure of manganese(II) glycolate dihydrate Acta Crystallographica Section B Structural Crystallography and Crystal Chemistry. ,vol. 36, pp. 701- 703 ,(1980) , 10.1107/S0567740880004190
Claudio Aguilar, Ulises Urzúa, Cecilia Koenig, Rafael Vicuña, Oxalate oxidase from Ceriporiopsis subvermispora: biochemical and cytochemical studies. Archives of Biochemistry and Biophysics. ,vol. 366, pp. 275- 282 ,(1999) , 10.1006/ABBI.1999.1216
Robert M. Esnouf, An extensively modified version of MolScript that includes greatly enhanced coloring capabilities Journal of Molecular Graphics & Modelling. ,vol. 15, pp. 132- 134 ,(1997) , 10.1016/S1093-3263(97)00021-1
P. G. Pietta, A. Calatroni, D. Agnellini, M. Pace, Improved Purification Protocol for Oxalate Oxidase from Barley Roots Preparative Biochemistry & Biotechnology. ,vol. 12, pp. 341- 353 ,(1982) , 10.1080/00327488208065681
R. A. Laskowski, M. W. MacArthur, D. S. Moss, J. M. Thornton, PROCHECK: a program to check the stereochemical quality of protein structures Journal of Applied Crystallography. ,vol. 26, pp. 283- 291 ,(1993) , 10.1107/S0021889892009944
Draženka Svedružić, Stefán Jónsson, Cory G. Toyota, Laurie A. Reinhardt, Stefano Ricagno, Ylva Lindqvist, Nigel G.J. Richards, The enzymes of oxalate metabolism: unexpected structures and mechanisms. Archives of Biochemistry and Biophysics. ,vol. 433, pp. 176- 192 ,(2005) , 10.1016/J.ABB.2004.08.032