Coding-noncoding gene expression in intrahepatic cholangiocarcinoma

摘要: Recent studies have shown that long noncoding RNAs (lncRNAs) play crucial roles in human cancers. However, the function of lncRNAs and their downstream mechanisms are largely unknown molecular pathogenesis intrahepatic cholangiocarcinoma (ICC). In present study, we performed transcriptomic profiling ICC paired adjacent noncancerous tissues (N) by using lncRNA messenger RNA (mRNA) microarrays. Quantitative real-time polymerase chain reaction was used to validate microarray results. We tested for correlations between expression levels target genes. Clinicopathologic characteristics overall survival were compared t test Kaplan-Meier method, respectively. A total 2773 significantly upregulated with tissues, whereas 2392 downregulated. Bioinformatic analysis indicated most genes involved carcinogenesis, hepatic system diseases, signal transductions. Positive found 4 lncRNA-mRNA pairs (RNA43085 SULF1, RNA47504 KDM8, RNA58630 PCSK6, RNA40057 CYP2D6). When clinicopathologic accounted for, cumulative rate be associated low CYP2D6 (P = 0.005) PCSK6 (P = 0.038). Patients high had a better prognosis (P = 0.014). Our results suggested is profoundly different from tissues. Thus, may potential diagnostic prognostic biomarker ICC. Furthermore, combined assessment mRNA expressions might predict patients