Retinal ganglion cells lose response to laminin with maturation

摘要: The decisive role played by adhesive interactions between neuronal processes and the culture substrate in determining form extent of neurite outgrowth vitro1,2 has greatly influenced ideas about mechanisms axonal growth guidance vertebrate nervous system. These studies have also helped to identify molecules that might be involved guiding vivo. One candidate molecule is laminin, a major gly-coprotein basal laminae3 which been shown induce wide variety embryonic neurones extend neurites culture4–8. Moreover, laminin found large amounts injured nerves can successfully regenerate but absent from where regeneration fails9–11. However, it unclear what regulate operate embryo when axon initiated. Here we examined requirements for vitro chick retinal ganglion cells, only cells retina send axons brain. We show while day 6 (E6) chicks profuse on those Ell do not, although they retain ability astrocytes via laminin-independent mechanism. This represents first evidence central system may undergo change their as mature.