Elevated levels of intracellular Ca2+ and apoptosis in human lung cancer cells given heat-shock.

摘要: The chronological changes in intracellular Ca 2+ concentrations ([Ca ] i ) were analysed during heat-induced apoptosis human lung cancer cell lines LK-2 (squamous carcinoma) and LU65A (large carcinoma). In cells, increased [Ca levels maintained at between 250-350 n m 9 h after heat-shock. Treatment with BAPTA, an chelator, prior to heat-shock, decreased the frequency of LK-2, while thapsigargin, a selective endoplasmic reticulum -ATPase inhibitor, did not change number apoptotic regardless presence or absence -supplemented medium. treatment BAPTA thapsigargin alter rates. Western blotting demonstrated that, although expression Bax Bcl-2 changed by p53 was elevated but cells. Immunohistochemistry showed that localized predominantly cytoplasms suggesting t...