Necrosis and apoptosis associated with distinct Ca2+ response patterns in target cells attacked by human natural killer cells.

摘要: 1. During the process of cell death, rises in cytosolic Ca2+ concentration ([Ca2+]i) together with structural changes were investigated target cells attacked by purified CD3-,CD16+ human natural killer (NK) cells. 2. In line K562, a rapid [Ca2+]i rise to 1-2 microM occurred few minutes after NK cell-target contact, immediately followed leakage indicator dye fura-2 from cell. Cells permeabilized, but their chromatin was not fragmented. The basically consistent those seen necrosis induced activated complement. 3. MOLT-4, which expressed apoptosis-inducing surface antigen Fas much more strongly than majority displayed 0.7-1 slow decline, often associated diminishing oscillations. As whole, remained higher 150 nM for at least 1.5-3 h (approximately 100 control cells). 4. MOLT-4 became bubble shaped within 20 min main reaching its peak, and then both fragmented into small pieces. These findings apoptosis monoclonal antibody against Fas. 5. lines insensitive presence an major histocompatibility complex class I (antibody-dependent cell-mediated cytotoxicity, ADCC). distinct responses patterns described above corresponded or different stimulated common ADCC pathway. 6. Human found be capable inducing (membrane damage) (nuclear depending on types. characteristic response profile good distinguishing between modes death cytotoxicity