The Botrytis cinerea PAK kinase BcCla4 mediates morphogenesis, growth and cell cycle regulating processes downstream of BcRac.
作者： Anna Minz-Dub , Amir Sharon
DOI: 10.1111/MMI.13642
关键词: Biology 、 Biochemistry 、 Effector 、 Morphogenesis 、 Cell cycle 、 Rac GTP-Binding Proteins 、 Mitosis 、 Cyclin-dependent kinase 、 Hyphal growth 、 Cell biology 、 Kinase
摘要: Summary Rac proteins are involved in a variety of cellular processes. Effector that interact with active Rac convey the GTPase-generated signal to downstream developmental cascades and Here we report on analysis main effector cascade BcRac, homolog grey mold fungus Botrytis cinerea. Several lines evidence highlighted p21-activated kinase Cla4 as an important fungi. Analysis Δbccla4 strains revealed BcCla4 protein was sufficient mediate all examined BcRac-driven processes, including hyphal growth morphogenesis, conidia production pathogenicity. In addition, had altered nuclei content, phenomenon previously observed Δbcrac isolates, thus connecting BcRac/BcCla4 module cell cycle control. Further analyses control mitotic entry through changes phosphorylation status cyclin dependent BcCdk1. The complete includes BcWee1, which is upstream These results provide mechanistic insight connection cycle, morphogenesis pathogenicity fungi, position most essential central regulator these processes BcRac.
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