The cdc25 phosphatase is essential for the G2/M phase transition in the basidiomycete yeast Ustilago maydis.

摘要: Summary Cdc25-related phosphatases reverse the inhibitory phosphorylation of mitotic Cyclin-dependent kinases mediated by Wee1-related kinases, thereby promoting entry into mitosis. In fission yeast, Schizosaccharomyces pombe, Cdc25 is required for mitosis, while in budding yeast Saccharomyces cerevisiae, Mih1 (the homologue Cdc25) not mitosis or viability. As these differences were linked to different cell division and growth mechanism species, we sought analyse roles Ustilago maydis, which as S. cerevisiae divides budding, but relies a polar growth. This basidiomycete perfectly suited relationships between cycle morphogenesis. We show that U. maydis contains single Cdc25-related protein, essential Loss function results specific G2 arrest correlated with high level Tyr15 Cdk1. Moreover, genetic interactions cdc25 wee1 clb2 support notion counteracts Wee1-mediated Cdk1–Clb2 complex. Our supports model Cdk1 primary operating at G2/M transition this fungus.