The role of proteolysis in cell cycle progression in Schizosaccharomyces pombe.

摘要: A cell-free system derived from Xenopus eggs was used to identify the 'destruction box' of Schizosaccharomyces pombe B-type cyclin, Cdc13, as residues 59-67: RHALDDVSN. Expression indestructible Cdc13 a regulated promoter in S.pombe blocked cells anaphase and inhibited septation, showing that destruction is necessary for exit mitosis, but not sister chromatid separation. In contrast, strong expression polypeptide comprising N-terminal 70 which acts competitive inhibitor box-mediated proteolysis, both separation whereas an equivalent construct with mutated box did not. Appropriately timed this fragment overcame G1 arrest seen cdc10 mutant strains, suggesting proteins required initiation S phase are subject by same proteolytic machinery cyclin.